U22-E05. Multi-use rooms for rodents and lagomorphs maintenance
Four multi-use rooms for maintenance of rodents and lagomorphs.
Lagomorphs can individually accommodate upto 102 rabbits.
+34 9340006100 -ext. 437807-info@nanbiosis.com
Four multi-use rooms for maintenance of rodents and lagomorphs.
Lagomorphs can individually accommodate upto 102 rabbits.
One barrier zone for rodents maintenance under SPF conditions with capacity for about 3,000 animals in microisolators. It has autoclaves, SAS to the material inlet, automatic beverage system by reverse osmosis, laminar flow hoods for animal handling and integrated operating room within the barrier zone.
One barrier zone for rodents maintenance under SPF conditions with capacity for about 3,000 animals in microisolators. It has autoclaves, SAS to the material inlet, automatic beverage system by reverse osmosis, laminar flow hoods for animal handling and integrated operating room within the barrier zone.
This unit has facilities with a perimeter area of 3,200 m2 and it has large rooms to house the experimental animals according to their species characteristics. Among the equipment and facilities include:
9 rooms for large animals (pigs) maintenance with capacity for about 350 animals, for long periods of time and environmental, nutritional and microbiological conditions controlled.
2 rooms for the maintenance of small ruminants (sheep and goats) with capacity for about 60 animals over long periods of time and environmental, nutritional and microbiological conditions controlled.
It houses different animal species, whether small or big (rats, mice, rabbits, dogs, pigs, sheep, etc), in compliance with the physical and legal conditions for experimental research.
Assays of cell viability/toxicity by XTT, release of LDH and similar indicators.
Internalization assays
Sample preparation, inclusion, section and staining for preclinical histology (paraffin and frozen samples) as well as anatomopathological evaluation.
Complete necropsy:
Product characterization studies, solubility and other properties studies; Inmunoassay techniques; Molecular biology techniques; Cell culture techniques; Flow cytometry; Safety studies: Nervous, cardiovascular and respiratory systems; Toxicology studies; PK/PD studies, Security testing of advanced therapies using small and large animals models.
Complete necropsy:
Leica TCS-SP5 confocal microscope with especial features that allows studying interactions between cells/tissues and materials. Indeed, the experience of the research group in charge of this Unit makes this a unique service for the study of cells and tissues and the interactions between various materials and cell components as well as between implants/scaffolds and tissues of the recipient organism. For example, it makes it possible to characterize in detail the specific markers of particular cell populations for tissue engineering applications and in vitro tests of biocompatibility of new materials, allowing the process of cell colonization of surfaces to be explored and simultaneous analysis of the cells or subcellular structures and materials. The combined use of fluorescence and reflection makes it possible to study the tissue-implant interface, with up to 8 fluorophores, at wavelengths from 405 to 633 nm allowing histological studies of a wide range of tissue types which would be restricted at certain fixed wavelengths due to autofluorescence phenomena.
Leica TCS-SP5 confocal microscope with:
DMI 6000 inverted microscope with 4 objectives:
10X (dry); 20X; 40X and 63X(for immersion).
Confocal module:
•• 3 channels for spectral detection
•• AOBS (Acousto Optical Beam Splitter)
•• Resonant scanning system.
4 lasers:
•• Argon laser (excitation at 458, 477, 488, 496 and 514 nm)
•• He/Ne laser (excitation at 633 nm)
•• DPSS (diode-pumped solid-state) laser (excitation at 561 nm)
•• UV laser (excitation at 405 nm)
Incubation chamber on a motorized stage.
Workstation with 4 types of software for data acquisition and processing:
•• 3D imaging
•• Colocalization analysis
•• FRAP (Fluorescence Recovery After Photobleaching)
•• FLIP (Fluorescene Loss In Photobleaching)
•• FRET (Fluorescence Resonance Energy Transfer)