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Nanbiosis

U7-E05. Wet Bench

• Wet Etching

Chemical bath system with several baths built into a safety cabinet made of anti-corrosive material (polypropylene). The cabinet is a closed unit and incorporates a ventilation system.
With the chemical bath system we can easily adapt the contents according to the requirements of the process, taking into account the polymer, resins or material to be etched.

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U7-E04. Laser Lithography (Mask Plotter)

• Laser Lithography (Mask Plotter)

•- Optical autofocus using 4 mm write head.
•- Minimum feature size: 1.0 µm (200 nm pixel size, 5.7 mm2/min writing speed).
•- Expose small parts samples up through 8” wafers, including masks blanks for photomask fabrication.
•- Diode laser: 405 nm wavelength, 50 mW max. power.
•- Back to front side alignment (alignment accuracy: 250 nm).
•- Grayscale exposure for 3D structures.
•- Stability of the system ensured by a climate chamber that provides constant temperature (±0.1°C).
•- Equipped with an interferometer stage for maximum alignment accuracy.
•- The conversion software accepts standard CAD formats: CIF, DXF and GDSII.

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U7-E03. UV optical Lithography (Mask Aligner)

• UV optical Lithography (Mask Aligner)

MJB4 Mask Aligner (SÜSS Microtech, Munich, Germany) is a precision instrument for high-resolution photolithography and is intended for use in research laboratories, small-series production and pilot projects.
The flexibility of the MJB4 is unsurpassed when exposing standard wafers and substrates and irregularly shaped substrates with a diameter of up to 100 mm or 4”x4” and various thicknesses.

• Hg lamp, 305nm to 450 nm wavelength and 350 W power.
• Soft-contact and hard-contact alignment. Adjusting range between 0 to 50 mm.
• 10x Optical microscope with diffraction reducing optics.
• 5” (125mm) Cr mask adapter available.
• Substrate size: from 10 mm to 4” (100 mm), thicknesses between 0.1 to 4 mm.
• Minimum resolution: 1um.
• Exposure modes: fixed power or fixed intensity.

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U7-E02. ToF-SIMS (Time of the Flight Secondary Ion Mass Spectroscopy)

• ToF-SIMS (Time of the Flight Secondary Ion Mass Spectroscopy)

ION-TOF IV (IONTOF, Münster, Germany) equipped with the state-of-the –art Bi cluster primary ion gun for analysis, and O2 and Cs ion guns for sputtering in the depth profiling mode.
Charge compensation capability using a pulsed electron-gun and/or O2 flood gas allows surface analysis and depth profiling of highly insulating samples
Large sample stage capable of holding wafers up to 3″ in diameter/width and 0.5″ in thickness. Samples sizes from few mm up to 10cm. Motorized sample stage that allows for automated analysis of large areas. Heating/cooling stage.

• High mass resolution, to distinguish species of similar nominal mass (mass resolution is at least 0.00x amu); Particles with the same nominal mass (e.g. Si2 and Fe, both with amu = 56 ) can be clearly distinguish.
• Mass range of 0-10,000 amu; ions (positive or negative), isotopes, and molecular compounds (including polymers, organic compounds, and up to amino acids fragments) can be detected.
• High sensitivity for trace elements or compounds, on the order of ppm to ppb for most species;
• Elemental and chemical mapping on a sub-micron scale;
• Depth profiling capabilities; sequential sputtering of surfaces allow analysis of elemental composition on materials from 1nm up to 10µm in depth (typical sputtering rates are ~10nm/minute).
• Retrospective analysis. Every pixel of a ToF-SIMS map represents a full mass spectrum. This allows an analyst to retrospectively produce maps for any mass of interest, and to interrogate regions of interest (ROI) for their chemical composition via computer processing after the dataset has been instrumentally acquired.
• Surveys of all masses on material surfaces; these may include single ions (positive or negative), individual isotopes, and molecular compounds;
• Surface analysis of insulating and conducting samples;

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U7-E01. Ebeam/SEM (Nova NanoSEM 230/Raith)

• Nova NanoSEM 230

›› High and Low Vacuum field emission Scanning
Electron Microscope (SEM ) incorporating:

•• Resolution: 1.6 nm at 1 kV (ETD and TLD detectors) in High Vacuum.
•• Resolution: 1.8 nm at 3 kV (LVD detectors) in Low Vacuum.
•• Field emission filament -> High-resolution images even of non-conductive samples.
•• Backscattered electron detector (vCD detector):
High-resolution images of backscattered electrons.
•• 6-channel preamplifier for detectors of solid state.
•• Compressor.
•• Cryo Can, anti-contamination system.
•• Thermoflex closed wáter circuit.
›› Electron beam lithography system with:
•• High writing resolution (maximum speed of 6 MHz and control of the dwell time < 2 ns). •• Electrostatic Beam Blanker. •• Picoammeter exchanger box. •• Keithley picoammeter. •• Advanced nanolithography system for microscope. •• Beam Blanker amplification electronics •• Software for correcting the Proximity Effect. •• Universal sample carrier for Lithography. •• EBL Starter kit.

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U14-S02. Flow Cytometry (Onsite&Remote) OUTSTANDING

Cellular phenotyping, cytokine assays, profiferation assays, viability and apoptosis.

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U14-S01. Cell cultures (Onsite&Remote) OUTSTANDING

Isolation and expansión of mesenchymal stem cells; viability assays and cytotoxicity, porcine mesenchymal stem cells (isolated from adipose tissue, bone marrow and synovial fluid) for preclinical trials. Murine mesenchymal stem (isolated from adipose and bone marrow).

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U14-E01. Cell culture rooms

Cell Culture Room 1:
Vertical Laminar Flow Cabinet Herasafe® (Formimetal).
CO2 incubator Heracell 150 (Thermo)
Nomarski phase inverted microscope and fluorescencia con cámara (Nikon)
Camera Color 1CCD (Nikon)
Cooled Camera black and white (Nikon)
Vacuum Pump ME4 (Vacuubrand)
Cell culture water Baths (Controltecnica)

Cell Culture Room 2:
Vertical Laminar Flow Cabinet Herasafe® (Formimetal)
CO2 incubator Heracell 150 (Thermo)
CO2 incubator Heracell 150 with CO2 control (Thermo)
phases inverted microscope with fluorescence (NIKON)
Vacuum Pump ME4 (Vacuubrand)
Cell Counter (Invitrogen)

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U14-E02. Molecular biology

Molecular Biology
Fume Hood (Formimetal)
Stereo microscope with camera and digital image set (NIKON)
light source (for the glass) (NIKON)
Refrigerated centrifuge for microtubs Biofuge® Pico (HERAEUS)
Digital circulation thermostatic bath (GRANT)
Thermostatic bath (for cell cultures temperar medios) (MEDIGEN))
Pipette controller pipette Powerpette-plus (IZASA)
Pipettes de volumen variable continuo with accessories (RAININ)
Vertical Freezer -20ºC (FIOCHETTI)
Freezer +2/+8ºC (CLASS ELECTRONIC)
Laboratory vertical freezer Herfreeze ®HFU-486-Basic (-80ºC) (HERAEUS)
Crioconservtion system with accessories
Liquid Nitrogen tank (taylor wharton)
Thermocycler (THERMO)
Sterilization incubator (Modelo UT ) (HERAEUS)
G-Box Translumitator and accessories (PC, Sofware…) (SYNGENE)
Electrophoresis system (IZASA)
Electrophoresis vertical jar
Electrophoresis horizontal (big) jar
Electrophoresis horizontal (small) jar
Rotary shaker with incubation (HEIDOLPH)
Magnetic stirrer with heating (ARE)
Analytical balance 210gr, 0,01 gr. (ACCULAB)
Precision analytical balance (OHAUS)
Ultrasonic water bath (SELECTA)
Crushed ice machine (Schottman)
Microwave (LG)
Pipette controller (POWERPETTE PLUS)
Thermoblock (Labolan)
Vortex 230 V, EU (VWR)
Freezer +4ºC/-20ºC
UV mini-1240 Spectrophotometer (SHIMADZU)
ELISA plate reader, Synergy MX (BIOTEK)
Flow cytometry FACSCalibur (BD)
pH-Meter (Symphony)
i-Blot (Invitrogen)
Autoclave

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U13-E02. Machines for mechanical tests

Machines for mechanical tests:

• Instron MicroTester 5848 and 5548: electromechanical machines for displacement or load control tests:

•• load range from 1mN to 2kN at full scale.
•• load cells of:+/- 5, 50, 500 and 1000N.
•• synchronous data capture in all the channels up to
500 Hz.
• Biaxial Instron MicroTester 8874: axial-torsional, servohydraulic testing machine:
•• load capacity of 25 kN and 100 Nm.
•• fatigue tests at a low number of cycles.

›› Software for data control and analysis.
›› Accessories and tools especially designed by the group, which allow a wide flexibility in designing tests, allowing the verification and/or validation of theoretical models.
›› AVE video extensometer connected to the machines; resolution of 0.5 μm. To determine the axial and transverse deformation without contact (tension, dynamic properties and viscoelasticity) of the samples.

• E1000 Test Instrument: Dynamic systems to offer slow-speed static testing and high-frequency dynamic fatigue testing with hundreds of Hertz capability: Load range: 250 N to 2.0 KN.
• Nano BIONIX MTS: For nanomechanical characterization of biomaterials. Load range to 0.001N to 0.5N.

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