In vivo Characterization of Nanomedicines

Efficacy studies of nanomaterial are conducted in animal utilizing a variety of models. We collaborate in the selection of the most suitable animal species and can developexperimental pathology in different animal models (large white pig, sheep, goat, beagle dog, minipig, rabbit, rat and mouse) upon request. At present we have some models of pathologies available:

*Marked (luciferase, GFP, others)

REFERENCES
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Ferrer-Font L, Arias-Ramos N, Lope-Piedrafita S, Julià-Sapé M, PumarolaM,Arús C, Candiota AP. Metronomic treatment in immunocompetent preclinical GL261 glioblastoma: effects of cyclophosphamide and temozolomide. NMR Biomed. 2017Sep;30(9). doi: 10.1002/nbm.3748. Epub 2017 Jun 1. PubMed PMID: 28570014.

Fernández, Y., Forarada, L., García-Aranda, N., Mancilla, S., Suárez-López, L., Céspedes, M. V., … Abasolo, I. (2015). Bioluminescent Imaging of Animal Models for Human Colorectal Cancer Tumor Growth and Metastatic Dissemination to Clinically Significant Sites. Journal of Molecular Biology and Molecular Imaging, 2(2), 1019–33.

Gener, P., Gouveia, L. P., Sabat, G. R., de Sousa Rafael, D. F., Fort, N. B., Arranja, A., … Schwartz, S. (2015). Fluorescent CSC models evidence that targeted nanomedicines improve treatment sensitivity of breast and colon cancer stem cells. Nanomedicine: Nanotechnology, Biology, and Medicine, 11(8), 1883–1892. https://doi.org/10.1016/j.nano.2015.07.009

Jakubowska, M., Śniegocka, M., Podgórska, E., Michalczyk-Wetula, D., Urbanska, K., Susz, A., … Plonka, P. M. (2013). Pulmonary metastases of the A549-derived lung adenocarcinoma tumors growing in nude mice. A multiple case study. Acta Biochimica Polonica. https://doi.org/10.1017/CBO9781107415324.004

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https://doi.org/10.1038/srep43702

Mateo, F., Meca-Cortés, Ó., Celià-Terrassa, T., Fernández, Y., Abasolo, I., Sánchez-Cid, L., … Thomson, T. M. (2014). SPARC mediates metastatic cooperation between CSC and non-CSC prostate cancer cell subpopulations. Molecular Cancer, 13(1), 237. https://doi.org/10.1186/1476-4598-13-237

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Thomas, R. C., Bath, M. F., Stover, C. M., Lambert, D. G., & Thompson, J. P. (2014). Exploring LPS-induced sepsis in rats and mice as a model to study potential protective effects of the nociceptin/orphanin FQ system. Peptides. https://doi.org/10.1016/j.peptides.2014.08.009

• Pharmacokinetic; Animal studies in small and big animals to determine ADME (absorption, distribution, metabolism and excretion) studies.
• Quantification of nanomedicines in fluids and biomarkers by ELISA, fluorescence and analytical techniques
• Validation and optimization of techniques
• Biodistribution by MRI, luminescence, CT or echography
• Studies in tissues by coupling hyperpolarization and MRI/MRS/MRSI at 7Tesla
• Metabolomics studies in biofluid by NMR (14T)

Molina I, Salvador F, Sánchez-Montalvá A, Artaza MA, Moreno R, Perin L, Esquisabel A, Pinto L, Pedraz JL. Pharmacokinetics of Benznidazole in Healthy Volunteers and Implications in Future Clinical Trials.Antimicrob Agents Chemother. 2017 Mar 24;61(4). pii: e01912-16. doi: 10.1128/AAC.01912-16. Print 2017 Apr.

References
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Rafael, D., Martínez, F., Andrade, F., Seras-Franzoso, J., Garcia-Aranda, N., Gener, P., … Schwartz, S. (2017). Efficient EFGR mediated siRNA delivery to breast cancer cells by Cetuximab functionalized Pluronic® F127/Gelatin. Chemical Engineering Journal. https://doi.org/10.1016/J.CEJ.2017.12.114