Synthesis of customized Nanoparticles for biomedical Applications

– Nanovesicular systems composed by sterols and cationic surfactants.
– Size in the nano-range (below 100nm) – Uniform shapes (spherical and unilamellar)
– Highly Monodisperse
– Long-term stability (up to one year)
– Antimicrobial and antifungal properties

– Drug Delivery Systems (DDS) for both pharmaceuticals (Active pharmaceutical ingredients (APIs), etc.) and biopharmaceuticals (proteins, DNA, RNAs, etc.)
– Entrapment of dyes, stabilizing and targeting moieties.

Ref:

1. New nanodrug improves the treatment of diabetic foot ulcers (http://www.dicat.csic.es/rdcsic/index.php/en/biologia-y-biomedicina-2/108-histories-d-exit/310-una-nanomedicina-mejora-la-regeneracion-de-las-ulceras-de-pie-diabetico), Patent WO 2014/019555 A1.
2. Multifunctional Nanovesicle-Bioactive Conjugates Prepared by a One-Step Scalable Method Using CO2-Expanded Solvents, Nano Letters, DOI: 10.1021/nl4017072.

– Nanovesicular systems composed by sterols and phospholipids
– Size in the nano range (below 200nm)
– Monodisperse
– Uniform shapes (spherical and unilamellars)
– Short-term stability (1 month)

– DDS for both pharmaceuticals and biopharmaceuticals.
– Entrapment of dyes, stabilizing and targeting moieties.

Ref:

1. Multifunctional Nanovesicle-Bioactive Conjugates Prepared by a One-Step Scalable Method Using CO2-Expanded Solvents, Nano Letters, DOI: 10.1021/nl4017072.

– PEGylated nanovesicular systems
– Size in the nano range (below 100 nm)
– Highly Monodisperse
– High Uniform shapes (spherical and unilamellar)
– Long term stability (up to one year)

– DDS for both pharmaceuticals and biopharmaceuticals, with high stability due to the functionalization with hydrophilic poly (ethylene glycol) (PEG), a stealth agent used to prolong blood-circulation time while reducing mononuclear phagocyte system uptake.
– Entrapment of dyes and targeting moieties.

Ref:

1. Multifunctional Nanovesicle-Bioactive Conjugates Prepared by a One-Step Scalable Method Using CO2-Expanded Solvents, Nano Letters, DOI: 10.1021/nl4017072

– Polymeric Nanoparticles composed by biocompatibles polymers, such as PVP, GANTREZ, EUDRAGIT, PEG, CYCLODEXTRINES.
– Size in the micro, submicron, and nano range
– Monodisperse
– Uniform shapes

-DDS for both pharmaceuticals (APIs, anticancer drugs,etc.)

Ref:

1. High Loading of Gentamicin in Bioadhesive PVM/MA Nanostructured Microparticles Using Compressed Carbon-Dioxide, Pharm Res (2011), DOI: 10.1007/s11095-010-0248-x
2. Novel bioactive hydrophobic gentamicin carriers for the treatment of intracellular bacterial infections, ActaBiomaterialia (2011), DOI:10.1016/j.actbio.2010.11.031

– Fine particles of APIs
– Size in the micro range
– Narrow size distribution
– High crystallinity degree
– High polymorphic purity
– Free of residual solvents

– To increase the bioavailability and dissolution rate of pharmaceuticals
– To allow the use of a more appropriate and/or convenient administration route, such as subcutaneous, intramuscular, topical and intestinal modes of administration

Ref:

1. Crystallization of Microparticulate Pure Polymorphs of Active Pharmaceutical Ingredients Using CO2-Expanded Solvents, Cryst. Growth Des. (2012), DOI: 10.1021/cg200356x

Gold nanoparticles with spherical shape and average diameters ranged between 60-80 nm

Application: Photothermal therapy and thermally induced gene expression

Ref:

– RSC Adv., 2016,6, 58723-58732.
– Biomaterials, Volume 35, Issue 28, September 2014, Pages 8134-8143
– Nanomedicine: Nanotechnology, Biology and Medicine, Volume 9, Issue 5, July 2013, Pages 646-656

Magnetic nanoparticles in the core, covered by a shell of SiO2 with an external layer of gold, and average diameter of 100 nm

Application: Photothermal therapy and MRI signaling (theranosticnanoparticles)

Ref:

– Nanoscale. 2014 Aug 7;6(15):9230-40.

Magnetic Gold-based nanoparticles with magnetic and optical properties. Spherical shape and average diameter of 150 nm

Application: Photothermal therapy and MRI signaling (theranostic nanoparticles)

Ref:

– Nanoscale. 2014 Aug 7;6(15):9230-40.

Gold nanoparticles with average diameters of 20 nm

Application: Transfection reagent

Ref:

– ActaBiomaterialia, Volume 7, Issue 10, October 2011, Pages 3645-3655

Gold-based nanorods

Application: Contrast agents for optical coherence tomography

Ref:

– ChemCommun (Camb). 2012 Jul 7; 48(53): 6654–6656

Gold-based nanorods

Application: Photothermal therapy

Ref:

– Materials Research Bulletin, Volume 48, Issue 10, October 2013, Pages 4051-4057

Copper sulfide nanoparticles with an average diameter of 150 nm

Application: Photothermal therapy

Ref:

– ACS Appl Mater Interfaces. 2016 Aug 24;8(33):21545-54.

Sulphur/copper hollow nanoparticles with an average diameter of 200 nm

Application: Photothermal therapy

Ref:

– ACS Appl Mater Interfaces. 2016 Aug 24;8(33):21545-54.

Carbon-based nanoparticles with spherical shape and average diameters ranged between 2-6 nm

Application: Bioimaging in the UV-Vis-Near Infrared ranges

Ref:

Chem. Eur. J. 10.1002/chem.201604216

Iron-based nanoparticles with spherical shape and average diameters ranged between 6 -13 nm

Application: MRI diagnosis (T2 contrast agents)

Ref:

J Nanopart Res (2014) 16:2292

Application: drug delivery

Ref:

– ActaBiomater. 2017 Mar 1;50:493-501.
– RSC Adv., 2016,6, 111060-111069

Application: Drug delivery and contrast agents (theranostics)

Ref:

Nanoscale, 2016,8, 6495-6506

– Nanovesicular systems composed by sterols and phospholipids
– Size in the nano range (below 200nm)
– Monodisperse
– Uniform shapes (spherical and unilamellar)
– Short-term stability (2 months)

– DDS for both pharmaceuticals and biopharmaceuticals, with high stability due to the functionalization with an RGD targeting peptide. – “Active targeting” through the incorporation of specific molecules on the outer surface of nanovesicles, which can provide more effective therapeutic action to a nanomedicine, due to a more specific and effective cellular uptake.

Ref:

1. α-Galactosidase A Loaded Nanoliposomes with Enhanced Enzymatic Activity and Intracellular Penetration, Nano Lett., 2013, DOI: 10.1002/adhm.201500746